Figure 1.
Distribution of αS in subcellular fractions in normal and tg mouse brains and transfected MES cells. (A) Scheme of the centrifugal fractionation procedure. (B) Endogenous αS distribution in normal mouse brain. Whole normal mouse brain was fractionated as in A and subjected to quantitative Western blotting with H3C Ab. Results are presented as the ratio of the amounts of αS monomer (17 kDa) in each fraction to that of total αS monomer in the starting homogenate (100%) run in parallel on each gel (means ± SD of three brains). (C) Higher portion of A53T than WT αS is associated with P25 microsomes. Results are presented as the ratio of the amounts of αS monomer in P25 to that in S370 analyzed in parallel. [Means ± SD of n = 5 brains and n = 8 MES cultures each for wt (empty bars) and A53T (filled bars).]