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. 2015 Sep 15;9(10):2063–2070. doi: 10.1016/j.molonc.2015.09.003

Figure 1.

Figure 1

The basis of the immunosequencing assay. Multiple V, D and J gene segments exist in the germline genome. Initial receptor diversity is generated by recombination of V, D and J segments; and additional non‐templated diversity is introduced at the junctions by insertion of random nucleotides (N). The immunosequencing assay uses a multiplex PCR with forward primers for each V segment and reverse primers for each J segment. A simplified TCRB locus is shown as an example.