Figure 2. Fine structure of differentiated organoid-derived monolayers.
(A) Monolayer culture derived from organoids from a duodenal biopsy of a control patient. Enterocytes show the typical morphology of fully polarized epithelial cells (light micrograph of a Toluidine blue–stained semi-thin section). (B) Electron micrograph of an organoid-derived monolayer from a control patient showing normal PAS staining, confined to intracellular glycogen and the glycocalix. Apical microvilli are cut transversally. (C) Electron micrograph of an organoid-derived enterocyte monolayer from FHL patient 3 shows a subapical accumulation of PAS-positive vesicles and virtually normal microvilli. Scale bar: 10 μm (A); 1 μm (B and C).