Figure 3.
Immunobead depletion and nanoparticle tracking analysis (NTA) profiles of syncytiotrophoblast extracellular microvesicles (STBMV) and syncytiotrophoblast extracellular exosomes (STBEX) pools from normal pregnancy (NP; n=3). A, Representative immunoblot showing endothelial nitric oxide synthase (eNOS) and placental alkaline phosphatase (PlAP) coexpression on STBMV pool (Total) and STBMV pulled out (PO) with anti-eNOS Dynabeads, anti-PlAP Dynabeads, anti-IgG2a Dynabeads (isotype control for eNOS), and anti-IgG1 Dynabeads (isotype control for PlAP). B, Representative NTA size vs number profiles of STBMV pool (solid line), supernatant from post-incubation with anti-eNOS Dynabeads (dashed line) and anti-PlAP Dynabeads (dotted line). C, STBMV pool (solid line), supernatant of post incubation with anti-IgG2a Dynabeads (dashed line) and anti-IgG1 Dynabeads (dotted line). D, Representative immunoblot showing eNOS and PlAP coexpression on STBEX pool (Total) and STBEX PO with anti-eNOS, anti-PlAP, anti-IgG2a, and anti-IgG1 Dynabeads. E, Representative NTA size vs number profiles of STBEX pool alone (solid line), supernatant from post-incubation with anti-eNOS (dashed line) and anti-PlAP (dotted line) Dynabeads. F, STBEX pool (solid line), supernatant of post incubation with anti-IgG2a (dashed line) and anti-IgG1 (dotted line) Dynabeads.