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. 2005 Mar;25(5):1620–1633. doi: 10.1128/MCB.25.5.1620-1633.2005

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FIG. 3. — Induction of IL-2Rα and IL-2 expression upon HIV infection. (A) Immunoblot analysis of total cell lysates from PBMCs (lane 1), activated PBMCs (lane 2), activated and HIV-1-infected PBMCs (lane 3), uninfected CEM-GFP cells (lane 4), and NL4.3-infected CEM-GFP cells (lane 5) using anti-IL-2Rα (upper panel) and anti-actin (lower panel). (B) RT-PCR analysis of PBMCs (lane 1), activated PBMCs (lane 2), activated and HIV-1-infected PBMCs (lane 3), uninfected CEM-GFP cells (lane 4), and NL4.3-infected CEM-GFP cells (lane 5) using primers specific for IL-2 (upper panel) and human GAPDH (hGAPDH, lower panel).