Figure 3.
(A) Timeline of the experimental procedure (n=9 for ND group and n=8 for intervention group). Eut, euthanasia; ND, normal diet. (B) Representative macroscopic photographs of livers. Arrowheads indicate HCCs. C, H&E stained liver sections from normal, NASH-HCC and NASH-HCC-cholestyramine mice at week 20. Bar = 70 μm. (D) Liver to body weight (%), fasting plasma glucose and liver TG levels at week 20. (E) Levels of inflammatory markers, liver tumor markers and mRNA expression of BA transporters at week 20. (F) Levels of DCA, TCA and TCDCA in liver at different groups. (G) Plasma LPS at week 20. (H) OUT numbers of Bacteroides and Clostridium in different groups at week 20. * p<0.05, ** p<0.01 vs. normal (n=9) (Mean ± SD).