Table 2. Chemokine/cytokine densitometric values ± s.e.m. from RayBiotech antibody array analysis of 1:10dilution of mouse serum.
| Treatment | ||||
|---|---|---|---|---|
|
| ||||
| Chemokine/cytokine | Control | Lean | DIO | Classification |
| Axl | 322.0±20.2 | 339.7± 23.75 | 292.0± 5.22 | Receptor |
| BLC | 334 ±11.98 | 337.3 ± 12.16 | 314.5 ± 25.38 | CXC chemokine |
| CD30L | 330.5 ± 12.48 | 329.6 ± 11.93 | 304.7 ± 11.55 | Receptor |
| CD30/TNFRSF8 | 327.5 ± 14.27 | 337.7 ± 9.96 | 294.0 ± 2.837 | Receptor |
| CD40 | 322.9± 14.83 | 325.1± 9.66 | 288.5± 8.60* | Receptor |
| CRG-2 | 325.6 ± 8.21 | 325.9 ±11.13 | 291.7 ± 10.09 | CXC chemokine |
| CTACK | 342.6 ± 12.54 | 329.1 ± 26.92 | 319.4 ± 14.32 | CXC chemokine |
| CXCL16 | 333.2 ± 6.72 | 342.2 ± 12.8 | 314.8 ± 7.65 | CXC chemokine |
| Eotaxin | 266.7 ± 6.17 | 319.1 ± 7.08* | 269.3 ± 9.1 | CC chemokine |
| Eotaxin-2 | 319.7 ± 17.65 | 327.1 ± 21.01 | 288.9 ± 3.88 | CC chemokine |
| FAS Ligand | 322.0 ±11.9 | 309.4 ± 4.6 | 301.3 ± 6.847 | Ligand |
| Fractalkine | 335.9 6.09 | 313.1 18.08 | 345.0 19.57 | CXC chemokine |
| G-CSF | 328.1 ± 4.79 | 339.4 ± 9.5 | 306.0 ± 7.55 | Growth factor |
| GM-CSF | 337.2 ± 7.45 | 345.2 ± 9.335 | 311.5 ± 5.81* | Growth factor |
| IFN-γ | 326.5 15.9 | 325.3 5.17 | 314.1 9.27 | Interferon |
| IGFBP-3 | 353.8 ± 17.75 | 417.3 ± 9.92* | 376.4 ± 6.08 | Binding protein |
| IGFBP-5 | 308.1 ± 5.76 | 349.1 ± 7.56* | 291.8 ± 5.201† | Binding protein |
| IGFBP-6 | 339.6 ± 3.42 | 391.6 ± 12.67* | 315.2 ± 5.01† | Binding protein |
| IL1-alpha | 353.5 ± 9.86 | 351.6 ± 29.65 | 317.1 ± 7.85 | Cytokine |
| IL1-beta | 345.9 ± 8.02 | 332.5 ± 18.45 | 310.9 ± 10.71 | Cytokine |
| IL-2 | 338.0 ± 8.27 | 328.1 ± 16.7 | 310.2 ± 10.95 | Cytokine |
| IL-3 | 336.8 ± 5.22 | 335.7 ± 11.23 | 343.9 ± 24.8 | Cytokine |
| IL-4 | 320.6 ± 14.42 | 342.9 ± 12.71* | 283.1 ±6.15† | Cytokine |
| IL-5 | 320.1 ± 10.00 | 342.5 ± 18.15 | 292.9 ±7.57*† | Cytokine |
| IL-6 | 321.6 ±4.67 | 318.5 ±10.02 | 345.1 6.90* | Cytokine |
| IL-9 | 328.0 ± 14.83 | 352.5 ± 13.54 | 313.5 ±6.70† | Cytokine |
| IL-10 | 319.1 ± 7.39 | 352.6 ± 10.65* | 302.5 ±4.98 | Cytokine |
| IL12-p40/p70 | 324.0 ± 9.89 | 354.9 ± 7.27* | 313.5 ±6.70† | Cytokine |
| IL12-p70 | 350.1 ± 12.19 | 364.3 ± 20.05 | 316.0 ±5.087† | Cytokine |
| IL-13 | 351.0 ±11.3 | 357.2 ± 11.73 | 313.1 ±5.39*† | Cytokine |
| IL-17 | 339.5 ± 10.53 | 340.2 ± 8.054 | 292.1 ±5.65*† | Cytokine |
| KC | 354.0 ± 10.4 | 326.2 ± 18.77 | 305.3 ± 8.12 | CXC chemokine |
| Leptin-R | 349.2 ±11.1 | 350.0 ± 7.055 | 291.2 ± 6.099*† | Receptor |
| Leptin | 307.7 ± 5.66 | 299.8 ±6.195 | 359.9 ± 11.86*† | Cytokine |
| LIX | 417.1 ± 7.01 | 386.5 ± 10.52* | 428.6.0 ± 7.013† | CXC chemokine |
| L-Selectin | 388.0 ± 15.17 | 353.4 ± 18.86 | 419.7 ± 7.86† | Adhesion molecule |
| Lymphotactin | 324.9 ± 9.41 | 306.6 ± 9.05 | 300.1 ± 7.71 | CC chemokine |
| MCP-1 | 331.1 ± 3.8 | 335.1 ± 18.5 | 315.9 ± 8.71 | CC chemokine |
| MCP-5 | 329.5 ± 5.47 | 368.2 ± 8.34* | 301.3 ± 8.93† | CC chemokine |
| M-CSF | 357.7 ± 6.02 | 364.1 ± 18.81 | 333.7 ± 11.05 | Growth factor |
| MIG | 320.0 ± 9.48 | 349.2 ± 28.6 | 296.4 ± 18.3 | CXC chemokine |
| MIP-1-alpha | 333.9 ± 7.13 | 355.8 ± 15.26 | 306.8 ± 6.60† | CC chemokine |
| MIP-1-gamma | 401.2± 7.84 | 402.5± 11.31 | 383.1± 5.39 | CC chemokine |
| MIP-2 | 394.2 ± 6.77 | 414.0 ± 8.63 | 366.4 ± 2.54*† | CXC chemokine |
| MIP-3-beta | 356.4 ± 15.39 | 381.7 ± 5.548 | 352.4 ± 5.35 | CC chemokine |
| MIP-3-alpha | 384.4 ± 16.91 | 343.4 ± 4.56 | 296.4 ± 7.79*† | CC chemokine |
| PF4 | 402.7 ± 7.5 | 416.3 ± 9.93 | 385.0 ± 7.34 | CXC chemokine |
| P-selectin | 410.5 ± 7.14 | 425.1 ± 11.64 | 384.4 ± 5.39*† | Adhesion molecule |
| RANTES | 332.7 ± 9.57 | 355.0 ± 11.05 | 297.5 ± 8.49† | CC chemokine |
| SCF | 269.8 ± 5.23 | 289.9 ± 7.25 | 267.3 ± 5.05 | Growth factor |
| SDF-1 | 269.3 ± 6.52 | 342.0 ± 14.41* | 258.8 ± 2.56† | CXC chemokine |
| TARC | 328.3 ± 8.38 | 333.1 ± 17.56 | 307.1 ± 7.26 | CC chemokine |
| TCA-3 | 339.3 ± 6.6 | 324.9 ± 13.73 | 306.0 ± 7.93 | CC chemokine |
| TECK | 333.5 ± 14.2 | 332.5 ± 6.46 | 315.5 ± 6.23 | CC chemokine |
| TIMP-1 | 318.8 ±11.5 | 353.8 ± 20.05 | 292.5 ± 4.44† | MMP inhibitor |
| TNF-α | 333.3 ± 5.71 | 350.3 ± 16.19 | 309.3 ± 4.79† | Growth factor |
| sTNF-RI | 339.5 ± 9.02 | 321.8 ± 12.73 | 334.5 ± 6.39 | Receptor |
| sTNF-RII | 346.8 ± 12.46 | 356.8 ± 8.86 | 352.1 ± 8.93 | Receptor |
| TPO | 351.0 ± 19.9 | 326.0 ± 15.61 | 340.5 ± 10.10 | Growth factor |
| VCAM-1 | 419.9 ± 8.15 | 410.1 ± 9.53 | 430.8 ± 4.87 | Adhesion molecule |
| VEGF | 315.8 ± 19.47 | 335.3 ± 26.18 | 283.3 ± 8.69 | Growth factor |
G-CSF = granulocyte colony-stimulating factor; GM-CSF = granulocyte monocyte colony stimulating factor; IL = interleukin; IFN-γ = interferongamma; MCP = monocyte chemoattractant protein; MCSF = Macrophage Colony Stimulating Factor; MIG = monokine induced by interferon gamma; PF4 = Platelet factor-4; SCF = stromal cell-derived cytokine; SDF = Stromal cell-derived factor-1; TARC = Thymus and activation-regulated chemokine; TCA = T-cell-activation protein; TECK = thymus expressed chemokine; TIMP = tissue inhibitor of metalloproteinase; TNF-α= tumor necrosis factor-alpha; TPO = Thrombopoietin; VCAM = vascular cell adhesion molecule-1; VEGF = vascular endothelial growth factor.
*
Significantly different from control. Bold faced values represent those statistically significant values.
†
Significantly different from lean.