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Rapid γH2AX association on the entire IgH locus in OHT-treated BL2-ΔC-AIDER cells. BL2-ΔC-AIDER cells were treated with OHT and used for ChIP experiment at indicated time points. Results of ChIP with anti-γH2AX (black) and nonspecific IgG (gray) are presented as intensity relative to input as described in Materials and Methods. Primer positions in the IgH gene locus are shown (Right). β2MG, β-2-microglobulin; VDJh5, rearranged VDJ; Cen., centromere; Tel., telomere; Chr., chromosome.
