TABLE 1.
Primers for quantitative PCRa
| Target | Sequences |
|---|---|
| TLR3 | |
| Forward | CCC AGC TCG ATG TTT CCT ACA |
| Reverse | CAG GCT TGG GAG ATA GGA GAA G |
| Probe | CAA CCT CCA TGA TGT CGG CAA CGG |
| TLR4 | |
| Forward | CAT GGA ACA CAT GGC TGC TAA |
| Reverse | GTA ATT CAT ACC CCT GGA AAG GAA |
| Probe | CTA TAG CAT GGA CCT TAC CGG GCA GAA GG |
| IFN-α | |
| Forward | CTG CTA GTG ATG AGC TAC TGG TCA A |
| Reverse | GGG TCA AGG CTC TCT TGT TCC T |
| Probe | CTG CTC CCT AGG ATG TGA CCT GCC TCA |
| IFN-β | |
| Forward | GCT CCT GGA GCA GCT GAA TG |
| Reverse | TCC GTC ATC TCC ATA GGG ATC T |
| Probe | TCA ACC TCA CCT ACA GGG CGG ACT TC |
| IFN-γ | |
| Forward | AGC CAG ATT ATC TCT TTC TAC CTC AGA |
| Reverse | GCA ATA CTC ATG AAT GCA TCC TTT |
| Probe | CAG GCC ATC AGC AAC AAC ATA AGG GTC |
| IL-4 | |
| Forward | CGC CAT GCA CGG AGA TG |
| Reverse | CGA GCT CAC TCT CTG TGG TGT T |
| Probe | TGC CAA ACG TCC TCA CAG CAA CGA |
| IL-6 | |
| Forward | CCA GAA ACC GCT ATG AAG TTC CT |
| Reverse | CAC CAG CAT CAG TCC CAA GA |
| Probe | TCT GCA AGA GAC TTC CAT CCA GTT GCC |
| IL-12p40 | |
| Forward | AGC TCG CAG CAA AGC AAG AT |
| Reverse | TGG AGA CAC CAG CAA AAC GA |
| Probe | TGT CCT CAG AAG CTA ACC ATC TCC TG |
| β-Actin | |
| Forward | CAC CGA TCC ACA CAG AGT ACT TG |
| Reverse | CAG TGC TGT CTG GTG GTA CCA |
| Probe | CAG TAA TCT CCT TCT GCA TCC TGT CAG CAA |
a
Probes labeled with FAM (5′) and TAMRA (6-carboxytetramethylrhodamine) (3′).