Fig. 2.
Histological evaluation of R-etodolac-treated TRAMP prostate tissues. Examples of ventral (a), dorsal (c), and lateral (e) lobes of the prostate in an untreated 30-week-old TRAMP mouse are shown. For comparison, histology sections of the ventral (b), dorsal (d), and lateral (f) prostate from an R-etodolac treated TRAMP mouse at 30 weeks of age are shown. Metastases in the lymph-nodes (g), lungs (i), and liver (k) of an untreated TRAMP mouse at 30 weeks of age are shown also. Examples of lymph nodes (h), lung (j), and liver (l)in R-etodolac treated TRAMP mice at 30 weeks of age are shown also. (m) Induction of tumor cell apoptosis by R-etodolac. For 2 weeks, we fed 6- to 7-month-old TRAMP mice with R-etodolac-supplemented chow or control chow, and they were then sacrificed. The prostate lobes were removed and frozen in OCT, sectioned, and subjected to TUNEL staining (red) and DAPI (to visualize nuclei) (×200). Extensive apoptosis (TUNEL-positive) was detected in the prostates of R-etodolac-fed TRAMP mice compared with control chow-fed mice.