Figure 5. MMTV-PyMT murine mammary tumor growth and metastasis are inhibited in PI3Kγ−/− mice.
3x106 MMTV-PyMT mammary tumor cells were implanted into the fourth mammary fat pad of PI3Kγ−/− (n = 4) and WT C57BL/6 mice (n = 6) and tumor growth was monitored for 5 weeks after tumor cell implantation. (A) Final tumor volume was determined by fluid displacement. (B) Number of lung metastases were counted from H&E stained sections of paraformaldehyde-fixed paraffin-embedded tissue of each perfused lung. (C) Number of immune cells (CD45+) in tumor tissue and lungs by FACS analysis. (D) Immune cell subsets in tumor tissue (left) and in lungs (right) by FACS analysis. All cell population calculated as % of immune (CD45+) cells. (E) Number of IFNγ+ CD8 cells in lungs and (F) number of TNFα+ CD8 and CD4 cells in tumor tissue and lungs. Number of cells was calculated by intracellular staining of a single cell suspension of tissue as described in M&M section. Statistics: two sample t-test.