Figure 4.
Bacteria promote distal colon inflammation and tumorigenesis in ApcMin/+;Il10−/− mice.
A–C) Colon macroscopic tumor counts (A), colitis scores (B), and small intestine tumor counts (C) from GF (n=7), SPF transferred (n=5) and SPF gavaged (n=7) ApcMin/+;Il10−/− mice. SPF transfer (transferred to SPF and allowed to naturally acquire their microbiota) and gavage (transferred to SPF and gavaged with the cecal and fecal contents from a wild type 129SvEv mouse) mice were sacrificed 16 weeks after transfer from GF. D) Colon H&Es from GF, SPF transferred, and SPF gavaged ApcMin/+;Il10−/− mice. Data are expressed as mean +/− SD. Two-tailed Mann-Whitney statistical analysis: ****p< 0.0001, ***p< 0.001, **p< 0.01, *p< 0.05, NS: not significant.