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SDS-PAGE of cell surface proteins. (A) Surface proteins of L. reuteri 100-23 extracted using a buffer containing SDS and 3-mercaptoethanol without (lane 1) and with (lane 2) sonication. The protein that was used for detection of the lsp gene is marked with an arrow. (B) Surface proteins of the wild type (lane 1) and the lsp mutant (lane 2) extracted with a buffer containing lysozyme and mutanolysin. Lsp is marked with an arrow.
