FIG. 5.
Protection against HIV-1 infection in vaccinated hu-PBL-NOD/SCID mice. Mice were immunized with HSV-transduced DC (HSV gp120MN/LAI or HSVlacZ in the vaccinated and control groups, respectively). Seven days later, the animals were challenged with infectious HIV-1 (strain HIV-1ADA or HIV-1LAI). Sera were then collected 14 days after challenge and analyzed by HIV-1 p24 ELISA or RT-PCR (to quantitate HIV RNA). Results are expressed as the ratio of viral load or nanograms per milliliter of HIV-1 p24 to the number of human CD45+ cells, as determined by flow cytometry. Open bars, data from individual animals; shaded bars, mean ± SEM of data from the two groups of mice. Top and bottom panels are from HIV-1LAI- and HIV-1ADA-infected animals, respectively. The experiment was set up with six to eight animals per group; samples from animals that did not reconstitute with human PBL were excluded from analysis. *, P < 0.05, calculated with Student's t test.