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Dynamics of YFP-PpAtg8 localization during macropexophagy in the wild-type and the Ppatg24Δ strain. After ethanol-adaptation for 15 min, both the wild-type (strain YAP0004) and the Ppatg24Δ (YAP2415) strains showed a ring structure representing a pexophagosome. After 3 h, while YFP-PpAtg8 fluorescence could be observed in the vacuolar lumen in wild-type cells, YFP fluorescence in the vacuolar lumen was not observed in the Ppatg24Δ strain.
