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. 2017 May 25;8:15522. doi: 10.1038/ncomms15522

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Copyright © 2017, The Author(s)

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(a) Western blot analysis of PHF5A levels in parental, PHF5A WT-expressing and PHF5A Y36C-expressing HCT116 cells. GAPDH is shown as a loading control. (b) Proliferation of parental, WT PHF5A-expressing or Y36C PHF5A-expressing HCT116 cells as measured by the Incucyte imaging system. X axis indicates hours post seeding, and y axis indicates percent of confluency. Error bar indicates s.d., n=5. (c) Western blot analysis of indicated SF3b complex protein levels following anti-SF3B1 pull-down from nuclear extracts containing WT or Y36C PHF5A. (d) Seventy-two hours growth inhibition profiling (CellTiter-Glo cellular viability assay) of parental, PHF5A WT-expressing and PHF5A Y36C-expressing HCT116 cells in response to indicated splicing modulators. Error bar indicates s.d., n=2.