Fig. 5.
Zymosan-induced protein extravasation, but not cellular infiltrates, are reduced in eNOS-/- mice and inhibitors of eNOS activation. Zymosan treatment increased white cell infiltration (A) and MPO activity (B) to the same extent in WT and eNOS-/- mice; however, the proteinaceous content (C) of the exudates was markedly reduced in eNOS-/- mice. In WT mice, administration of LY294002 (20 μg per pouch) or GA (20 μg per pouch) did not modify zymosan-induced cellular infiltration (D) but inhibited protein extravasation (E). DMSO is used as a control as above. Data are expressed as mean ± SEM. ***, P < 0.001; n = 5 mice per group for A-C and n = 8 per group for D and E.