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Incubation of BPN-15606 (lanes 1–6) with H4-APP751 neuroglioma cells transfected with the Myc-tagged Notch (NΔE) construct does not inhibit Notch proteolysis. Alternatively, incubation with δ-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (lanes 7–10) potently inhibits Notch proteolysis.
