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. 2004 Dec 27;102(1):63–68. doi: 10.1073/pnas.0406424101

Fig. 2.

Fig. 2.

The progression of HTLV-I-transformed cells through S/G2/M is delayed. (A) Jurkat- and HTLV-I-transformed MT4, C8166, and C91PL cells were synchronized at the G1/S boundary by hydroxyurea treatment (Materials and Methods). After release from the G1/S arrest, cells were collected and subjected to flow cytometry at the times indicated (0, 4, 6, 8, 10, and 12 h after release). The fractions of cell population in G1, S, and G2/M phases of the cell cycle are indicated (in black, gray, and white, respectively). (B) The DNA profiles of the aforementioned T cell lines (from left to right: Jurkat, MT4, C8166, and C91PL) at 12 h after release are shown. The percentages of cells in G1, S, and G2/M are indicated. (C) Jurkat and MT4 cells were arrested at the G1/S border by hydroxyurea, released from the arrest, and, at the indicated time after release (0, 4, 6, 8, 10, and 12 h), collected for flow cytometry (Left) and immunoblots using cyclin B1, securin, and Tax antibodies (Right).