TABLE 2.
PCR specificity test with E. coli O145 wzx and wzy genesa
| Gene | Base positions of the genes | Primer name, base positions, and sequences
|
|
|---|---|---|---|
| Forward primers | Reverse primers | ||
| wzx | 4369-5607 | wl-2131 (4468-4488), 5′-CCATCAACAGATTTAGGAGTG-3′ | wl-2132 (5059-5077), 3′-CTATCTAAGCGCCATCTTT-5′ |
| wl-2133 (5054-5074), 5′-CGTTTGATAGATTCGCGGTAG-3′ | wl-2134 (5532-5552), 3′-ACCATACTATCGATCAAAACA-5′ | ||
| wzy | 5615-6802 | wl-2135 (6061-6078), 5′-TGCCACTGATGGGATTAG-3′ | wl-2136 (6687-6706), 3′-ATAGGCCCGAAAGTTTAAGT-5′ |
| wl-2137 (5849-5866), 5′-GTTGCTTCAGCCCTTTTC-3′ | wl-2138 (6280-6297), 3′-TCTGTCACGGTCGTAAGC-5′ | ||
a
The correct PCR products were obtained in the pool containing E. coli O145 DNA with all of the four primer pairs. No bands were obtained in any other pools.