FIG. 8.
Effect of hCAP-D2 depletion on the metaphase plate. Cells were treated with control or hCAP-D2 siRNA and analyzed by immunofluorescence after a 72-h recovery. (A) Example of normal (a) and abnormal metaphase pictures obtained in hCAP-D2-depleted cells (b to d). Cells were immunostained with antitubulin antibodies (red) and counterstained with DAPI (blue). (B) Cells treated with control (left) or hCAP-D2 (right) siRNA were double stained with anti-Aurora B and anti-hCAP-D2 antibodies and counterstained with DAPI. Cells in prometaphase (a to a" and c to c") or metaphase (b to b", d to d", and e to e") are shown.