FIG. 5.
Characterization of parafibromin truncation mutant constructs. (A) Schematic diagram of parafibromin truncation mutant constructs and the L64P mutant construct representing germ line alterations identified from HPT-JT patients. (B) 293T cell lysates from cells transfected with Flag-tagged WT parafibromin or Flag-tagged mutant forms of parafibromin were immunoblotted with anti-Flag antibodies. (C) Immunoprecipitations (IP) with anti-Flag antibodies from 293T cell lysates cotransfected with either hLeo1 (left), hPaf1 (middle), or hCtr9 (right) together with the indicated Flag-tagged WT parafibromin or Flag-tagged parafibromin mutant constructs. Transfectants were immunoblotted with anti-hLeo1, anti-hPaf1, or anti-hCtr9 antibodies (top) or with anti-Flag antibody (bottom) as shown. Untransfected (UT) 293T cells were used as a negative control.