FIG. 5.
Direct telomerase assay with hTER template mutant. Telomerase extension was primed by oligonucleotide tel3.0 (lanes 1 to 8) or oligonucleotide TS-C (lanes 9 to 16) after incubation of the primer with POT1 dialysis buffer (lanes 1, 5, 9, and 13), 124 nM POT1 (lanes 2, 6, 10, and 14), 247 nM POT1 (lanes 3, 7, 11, and 15), or 425 nM POT1 (lanes 4, 8, 12, and 16). Reactions were initiated by addition of in vitro-translated hTERT reconstituted with wt hTER (lanes 1 to 4 and 9 to 12) or C51G template mutant hTER (lanes 5 to 8 and 13 to 16). +1 indicates the position of the first incorporated nucleotide. Asterisks indicates RNase A-insensitive bands likely stemming from non-telomerase-directed nucleotide addition. Lower panel, B-10mer loading control (LC).