Fig. 5.
The chronic EGFR activity maintains ERK1/2 in its phosphorylated state. A, Preovulatory follicles were incubated with LH for 1 h, at which time AG1478 or vehicle was added for 15, 30, and 90 min. At the end of the incubation period, the follicles were frozen. As a positive control, AG1478 was not added to the incubation medium and as negative controls, ovarian follicles were incubated without LH or preincubated with AG1478 for 1 h before the addition of LH (lane 2). B, Densitometric quantification of the results normalized against gERK1/2 protein levels. The histograms show means ± se of three independent experiments (*, P < 0.05 vs. corresponding LH treatment).