Figure 2. C3, Masp1 and Masp2 knockdown affect neuronal migration.
(a–e) Brains were electroporated in utero (E14–E18) with control shRNA (a, n=4), C3 shRNA (b, n=5), Masp1 shRNA (c, n=5) or Masp2 shRNA (d, n=4). The scale bars are 50 μm. (e) The position of GFP+ neurons across the width of the cortex was analysed and is shown in five bins (from the VZ to the CP). All shRNA treatments were compared to control shRNA. (f–n) Postnatal positioning and the identity of C3, Masp1 and Masp2 knockdown cells. Brains electroporated in utero on E14 with control shRNA (f,j), C3 shRNA (g,k), Masp1 shRNA (h,l) or Masp2 shRNA (i,m) were immunostained at postnatal day 8 (P8) with anti-CUX1 or anti-TBR1 antibodies (f–i,j–m, respectively). Black and red boxes show the position of the enlargements underneath each slice. The immunostainings for the enlarged areas are shown together with GFP or with the outlines of GFP-positive cells. (n) The position of GFP+ neurons across the width of the P8 cortex was analysed and is shown in five bins (from the VZ to the CP). All shRNA treatments were compared to control shRNA. The scale bars are 50 μm. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, histograms present means±s.e.m.