Figure 1.
Docetaxel induces formation of giant MP cells.(A) Cell-cycle histograms of doubly stained PC-3 cells treated with docetaxel at 5 nM concentration for different time points showing mitotic arrest and slippage at different time points. (B) Representative immunofluorescent confocal micrographs of PC-3 cells treated with docetaxel for 24 and 72 h, indicating mitotic arrest and emergence of giant MP cells respectively. Centrosomes and microtubules were immunolabeled for γ-tubulin (green) and α-tubulin (red), respectively, and DNA was counterstained with DAPI (blue). Scale bar (white), 5 μm. (C) Cell-cycle histogram of docetaxel treated PC-3 cells showing emergence of polyploid population. (D) Bar-graphs showing the percentage of sub-G1 and mitotic population resulting from 5 nM docetaxel treatment. (E) Bar-graphs showing the percentage of giant MP cells 72 h after docetaxel treatment.