Fig. 2.
Detection of antigen-responsive T cells using the DNA barcode-labeled MHC multimer methodology. A PE-labeled dextran backbone carrying a number of SA binding sites (illustrated as an X) is applied to co-attach biotinylated molecules; DNA barcodes and pMHCs. Thus >1000 pMHC multimers can be generated, each carrying a different DNA barcode. All MHC multimer binding T cells are sorted based on the common fluorescent label and the associated DNA barcodes are amplified and sequenced. The relative numbers of DNA barcode reads are used to determine the composition of antigen-responsive T cells in the sample