Fig. 2.
Pharmacologic ascorbic acid targets reactive oxidative species and labile iron pool. (A) OCI-MY5 WT cells were incubated with or without catalase (100 U/mL), NAC (15 mM) or DFO (200 μM) for 3 h following treatment with PAA. PAA was washed away after 1 h treatment and cell viability was determined 24 h later. (B) EV OCI-MY5 and OE-Fpn1 OCI-MY5 were treated with or without PAA (0–20 mM). PAA was washed away after 1 h and cell viability was measured 24 h later. EV OCI-MY5 (C) and OE-Fpn1 OCI-MY5 (d) were incubated with or without iron (Fe-NTA (FE), 100 μM). After 18 h cells were treated with or without DFO (200 μM) for 3 h followed by PAA treatment for 1 h and cell viability was measured as described in A.