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. 2017 Apr 18;12:3153–3170. doi: 10.2147/IJN.S133430

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© 2017 Meyer et al. This work is published and licensed by Dove Medical Press Limited

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Quantification of exosome uptake in HEK293 cells by flow cytometry.

Notes: Recipient cells were loaded with either fVSVG-RFP or RFP-mVSVG pseudotyped exosomes for 48 hours, then washed and subjected to fluorescence-activated cell-sorting analysis. Right shifts in fluorescence signals for both fVSVG (C) and mVSVG (B) exosomes are shown in comparison with the negative control (A) in panel (D), indicating an enhancement (~9.7-fold) in exosome uptake by pseudotyping in HEK293 cells.

Abbreviations: VSVG, vesicular stomatitis virus glycoprotein; fVSVG, full-length VSVG; mVSVG, minimal VSVG.