Fig. 4.
PTH genes in zebrafish. (A) A phylogenetic tree of PTH and PTHrP genes in vertebrates. Dr, zebrafish (Danio rerio); Gg, chicken (Gallus gallus); Hs, human (Homo sapiens); Mm, mouse (Mus musculus); Sa, seabream (Sparus aurata); Tr, fugu (Takifugu rubripes). (B) Comparison of the partial peptide sequences of zebrafish PTH and amniote PTH. The N-terminal 34 aa of mature human PTH peptide are sufficient for the biological activity of PTH. This alignment includes the N-terminal amino acids (1–34) with 2 aa before the final proteolytic cleavage site. (C) Gills in adult zebrafish express both the PTH genes and CasR. PTH and CasR were amplified from adult brain and gills by RT-PCR. Arrowhead indicates cDNA product.