Fig. 6.
Effect of Foxo1 and Foxo3 inactivation upon insulin-regulated hepatic gene expression in mouse primary hepatocytes. A, Effect of insulin on Foxo1 and Foxo3 protein degradation. Mouse primary hepatoctyes isolated from the liver of 12-wk-old control (CNTR), F1KO, F3KO, and F1/3KO mice were cultured and treated were treated with 100 nm insulin for 0.5 h and then protein lysastes analyzed by immunoblotting against antibody of Foxo1, Foxo3, or β-actin. B and C, Effects of insulin on Pck1 and G6pc (B) and Fasn, and Hmgcr (C) gene expression in primary hepatocytes deficient in Foxo genes. The primary hepatocytes from A were cultured and treated with 100 nm insulin for 6 h. Then RNA was extracted and levels of gene expression were analyzed by real-time PCR, and data are presented in comparison with control. *, P < 0.05 indicates the difference between basal level and insulin treatment; #, P < 0.05 indicates the difference between control and other groups without insulin treatment (n = 3 different experiments).