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. 2017 Apr 7;8:14907. doi: 10.1038/ncomms14907

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Copyright © 2017, The Author(s)

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(a–d) Immunocytochemistry for Gpsm2 (a,b) or Gα_i3 (c,d) and staining with phalloidin (Ph, magenta) shows protein localization at the tips of stereocilia in IHCs from shaker 2 (sh2/+, a,c) and whirler (wi/+, b,d) control mice at P8. Homozygous mutations (sh2/sh2 and wi/wi) lead to a loss of Gpsm2 and Gα_i3 stereocilia tip staining. The immunostainings were repeated four times. (e,f) Immunocytochemistry for myosin 15 (green) and staining with phalloidin (Ph, magenta) shows protein localization at tips of stereocilia in IHCs from controls of Gpsm2 (e) and Gnai3 (f) cKO P8 mice. Myosin 15 protein is still present at the tip of shortened stereocilia of both cKOs, though in reduced amounts. (g,h) Immunocytochemistry for whirlin (green) and staining with phalloidin (Ph, magenta) shows protein localization at tips of stereocilia in IHCs from controls of Gpsm2 (g) and Gnai3 (h) P8 mutant mice. Gpsm2 and Gnai3 mutation leads to a strong reduction of whirlin stereocilia staining. Scale bars, 4 μm. The immunostainings (e–h) were repeated six times and imaged blindly.