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. Author manuscript; available in PMC: 2017 Apr 4.
Published in final edited form as: Nature. 2016 Jan 6;529(7585):216–220. doi: 10.1038/nature16498

Figure 4. MYC is a critical component of FOXO1 signalling in ECs.

Figure 4

a,b, ECAR (a) and OCR (b) in MYC siRNA- (siMYC) or scrambled siRNA- (siSCR) transfected ECs (ECAR: n = 5; OCR: n = 5). Data represent mean ± s.d., two-tailed unpaired t-test. c, Staining for MYC , VECAD and PECAM in retinas of MyciEC-KO and control mice. d,e, Images of IB4- (d) and IB4- and ERG- (e) stained P5 retinas of control and MyciEC-KO mice. f,g, Images of IB4- (f) and pHH3- and IB4- (g) stained P5 retinal vessels in MyciEC-OE and control mice. h,i, Representative images (h) and quantification (i) of IB4-stained P5 retinas in control, Foxo1iEC-CA, MyciEC-OE and Foxo1iEC-CA/MyciEC-OE double mutants (n ≥ 6). j,k, ECAR (j) and OCR (k) in AdCTL, AdFOXO1CA, AdFOXO1CA/AdMYC and AdMYC-transduced HUVECs showing restoration of metabolic activity in FOXO1CA/MYC co-expressing ECs (ECAR: n = 8; OCR: n ≥ 3). Data in i-k represent mean ± s.d., one-way ANOVA with Bonferroni’s multiple comparison post-hoc test. **P < 0.01; ***P < 0.001; ****P < 0.0001.