Figure 2. Sensitivity of RMS cell lines to innate immune stimuli in the presence of LCL161.
A. Kym-1 cells were treated with vehicle control (DMSO) or 10 nM LCL161 and the following innate immune stimulants: BSA control (100 ng/mL), VSVΔ51-GFP (MOI 0.1), IFNγ (1000 U/mL), or TWEAK (100 ng/mL). B, C, D. Alamar Blue viability assays were performed after 24 h. LCL161-resistant RMS cell lines (RH36, RH41, and RD) were treated with DMSO or 5 μM LCL161 and innate immune stimulants at the same concentrations as stated above, followed by Alamar Blue viability assays after 24 h (B, C, and D, respectively). E. Alamar blue viability assays of Kym-1 cells treated with vehicle or 10 nM LCL161 and increasing MOI of VSVΔ51-GFP for 24 h. F-H. RH36, RH41, and RD cells were treated with vehicle or 5 μM LCL161 and increasing MOI of VSVΔ51-GFP for 24 h, followed by Alamar blue viability assays. (*,p < 0.05).