Table 2. Rate constants and permeability to CH3NH2/NH3 of human RBC ghosts.
| Controls
|
Variants
|
||||||
|---|---|---|---|---|---|---|---|
| Substrate | RhD-positive (n = 3) | RhD-negative (n = 3) | Rhnull regulator of P1-P4 | Rhmod of P5 | Rhnull amorph of P6 | LWnull of P7/P8 | 4.2null of P11 |
| CH3NH2 | |||||||
| k, s-1 | 0.95 ± 0.1 | 0.81 ± 0.03 | 0.14 ± 0.03* | 0.26 | 0.38 | 0.8/0.8 | 0.74 |
| P′, cm·s-1 | 0.88 ± 0.09 | 0.71 ± 0.03 | 0.12 ± 0.03* | 0.23 | 0.36 | 0.8/0.7 | 0.68 |
| NH3 | |||||||
| k, s-1 | 4.95 ± 0.28 | 5.4 ± 0.28 | 2.48 ± 0.09* | 2.6 | 2.9 | 3.9/4.1 | 4.56 |
| P′, cm·s-1 | 4.46 ± 0.25 | 3.91 ± 0.21 | 2.54 ± 0.08* | 2.63 | 2.53 | 4.1/4.3 | 4.11 |
Figures indicate alkalinization rate constants (k) and permeability (P′ × 10-4) to CH3NH2/NH3 of ghosts submitted to 32.5 mM MA or ammonium gradient.
*
, P < 0.001 versus RhD-positive and RhD-negative.