Figure 4. IL-6 upregulation is responsible for VM promotion in glioma cells.
(A, B) Glioma cells were treated with DMEM medium or M2-CM in the presence or absence of anti-IL-6 at 1 μg/ml, or isotype-matched IgG (IgG) control for 24 hours. Representative images (A) and quantification (B) of tubule formation assay on Matrigel matrix in vitro (×100). (C) As previously treatment, the protein levels of VM markers were then detected by Western blotting. (D, E) Glioma cells were treated with rhIL-6 in a concentration of 100 ng/mL or PBS control for 24 hours. Representative images (D) and quantification (E) of tubule formation assay on Matrigel matrix in vitro (×100). (F) VM markers protein of glioma cells, alone or after cultured with rhIL-6. Each bar represents the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.