Figure 5. Hypoxia induced CCL28 upregulation is HIF1α dependent.

A. SK-Hep-1 cells transfected with HIF1α-siRNA (siHIF1α) or scramble control. Then western blot assay was performed. B. and C. RT-qPCR and ELISA analysis of CCL28 expression in SK-Hep-1 cells transfected with HIF1α-siRNA (siHIF1α) or scramble control. D. HepG2 cells transfected with HIF1α-siRNA (siHIF1α) or scramble control. Then western blot assay was performed. E. and F. RT-qPCR and ELISA analysis of CCL28 expression in HepG2 cells transfected with HIF1α-siRNA (siHIF1α) or scramble control. G. Hep 3B cells transfected with HIF1α-siRNA (siHIF1α) or scramble control. Then western blot assay was performed. H. and I. RT-qPCR and ELISA analysis of CCL28 expression in Hep 3B cells transfected with HIF1α-siRNA (siHIF1α) or scramble control. J. Hepa1-6 cells transfected with HIF1α-siRNA (siHIF1α) or scramble control. Then western blot assay was performed. K. and L. RT-qPCR and ELISA analysis of CCL28 expression in Hepa1-6 cells transfected with HIF1α-siRNA (siHIF1α) or scramble control. The data are shown as the means ± SD. *, P < 0.05, * *, P < 0.01.