Figure 1. Transduction of organotypic explant of murine cochlea with natural AAV serotypes and Anc80L65.
Representative confocal projections of an in vitro comparison of several AAV serotypes for eGFP transgene expression in cochlear explants of C57BL/6 mice. A–G: Expression at the cochlear base for all serotypes, and apex and base for Anc80L65 after incubation with 1010 genome containing (GC) particles for 48 hours. Scale bar = 100 μm. (Top: Myo7A+TuJ1, bottom: eGFP only, middle: overlay, S. Cells: Supporting Cells, OHC: Outer Hair Cell, IHC: Inner Hair Cell) H–K: Percentage of eGFP-positive hair cells per 100 μm after 48h or 48h+5 days of incubation. Mean indicated by horizontal bar. Each condition had minimally N=3 for the 48h, and N=2 for the 48h+5d unless otherwise noted.