Figure 2. Altered enzymatic activity in PH platelets.
(A) Enzymatic activity of PDH in platelets from control (n = 7) and PH (n = 9) subjects. Data are mean ± SEM. (B) Representative Western blots for PDK1 and integrin αIIβ expression in platelets from control and PH subjects. (C) Quantification of PDK1/integrin αIIβ protein expression in platelets from control (n = 17) and PH patients (n = 17). (D) Representative Western blots for complexes (Cx) I, II, IV, and V and integrin αIIβ expression in platelets from control and PH subjects. (E) Quantification of complex/integrin αIIβ protein expression in platelets from control (n = 8) and PH patients (n = 6). (F) Enzymatic activity (fold-change of control) of platelet mitochondrial Cx I, II, and IV and citrate synthase (CS) from control (n = 12) and PH (n = 17) subjects. Boxes show the 25%–75% percentile; whiskers show the minimum-maximum; lines represent the median; + represents the mean. Unpaired 2-tailed t test was used to compare groups. *P < 0.01.