Figure 8. C3(H2O) uptake modulates the cytokine profile of activated CD4+ T cells.
Supernatants from CD4+ T cells incubated for 18 hours on anti-CD3– (mAb OKT-3), anti-CD46– (mAb TRA-2-10), anti-CD3 plus anti-CD46–, or nonactivated-coated (NA) (MOPC-31) plates in the presence of C3(H2O), C3b, or media only were assessed for IL-6, IFN-γ, TNF, and IL-10 by CBA. Experiments with C3b were only performed with NA and α-CD3+α-CD46 activated cells. Data are expressed as mean ± SEM. n = 6 from 2 healthy donors in 2 independent experiments run in triplicate. ***P < 0.001 by 1-way ANOVA with Bonferroni’s multiple comparison test.