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Distinct patterns of G1/S and G2/M cell cycle regulation of E2F-dependent genes. (A) FACS analysis of REF52 cells that were synchronized by HU block, and then released into the cell cycle. (B) Northern analysis of G2/M gene and G1/S gene expression in HU-synchronized REF52 cells. Approximately 2 μg of mRNA was loaded in each lane. Expression of GADPH was evaluated to confirm equal loading.
