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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Dec 17;1860(3):588–598. doi: 10.1016/j.bbagen.2015.12.012

Figure 2. Deficiency of Syk elevates the production of IFNβ and IL-10, but suppresses the levels of TNFα and IL-6 in LPS-stimulated dendritic cells.

Figure 2

Cell-free supernatants were collected from wild type and Syk deficient dendritic cells stimulated with LPS for 24 h, and the production of (A) IFNβ, (C) IL-10, (D) TNFα, and (E) IL-6 was determined by ELISA. The mRNA levels of IFNβ (B) and other cytokines (F) from wild type and Syk deficient cells stimulated with LPS (1 μg/ml) for the time indicated were examined by RT- PCR; TLR3 (Poly I:C LMW; 1 μg/ml) and TLR9 (ODN1826; 10μg/ml) agonists were used to stimulate wild type and Syk deficient BMDCs for 24 h, and the production of IFNβ (G) and other cytokines (H) measured by ELISA, “*” and “***” indicates P<0.05 and P<0.001, respectively. Data represents the mean ± S.D. of three biological replicates.