Figure 4.
Rot-induced ROS activates NF-κB in CECs and enhances TNT formation via upregulation of TNFαip2. (a) Western blotting showing the level of p-IκB, TNFα and TNFαip2 in different groups. (b) p-IκB and TNFαip2 expression induced by Rot was strikingly decreased in hCECs with SC-514. (c) Quantification of p-IkB intensity. The levels of phosphorylation were normalized to β-Actin. Results were obtained from three independent experiments (*P<0.001 versus UT; #P<0.05 versus Rot). (d) hCECs were treated with Rot, Rot plus NAC or Rot plus SC-514 for 12 and 24 h; the number of Filiopodia outgrowth per cell was calculated (*P<0.001 versus UT; #P<0.05 versus Rot, n=100). (e) The proposed mechanism whereby Rot-induced ROS activates NF-κB and enhances TNT formation via upregulation of TNFαip2 in hCECs