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. 2017 Jan 24;7:41122. doi: 10.1038/srep41122

Figure 1. Foxj1CreERT2-tdT (Foxj1-tdT) expression is confined to molecularly confirmed ependymal cells in uninjured adult murine spinal cord.

Figure 1

(a1–5, b1–4) Single channel and merged immunofluorescence images of transverse (a) or horizontal (b) sections through uninjured spinal cord ependyma (Ep) and central canal (CC). (a1-a5) Note that all ependymal cells with apical membranes (A) in contact with the CC lumen express Foxj1-tdT in those apical membranes and adjacent cytoplasm (A) and these Foxj1-tdT cells also express vimentin (Vim) in their central and basal cell portions and in some radial processes. (b1-b4) Note that all ependymal cell apical membranes (A) in contact with CC lumen are intensely co-labeled with both Foxj1-tdT and CD133 (arrows), which is also present but less intense in adjacent apical cytoplasm (A). (c) Graph comparing the percent of overlap of Foxj1-tdT and CD133 in the ependymal cell layer. n = 4 per group, *p < 0.001 (t-test). (d–g) Transverse and horizontal images showing Foxj1-tdT labeled ependyma and GFAP-immunoreactive astrocytes uninjured spinal cord. Note the complete absence of Foxj1-tdT labeled cells from all regions of grey (GM) and white matter (WM) and that no astrocytes express Foxj1-tdT.