Figure 7. miR-195 is downregulated in CCA cells.
A: qRT-PCR for 20 human CCA tissues and paired normal liver specimens is shown. MiR-195 expression is lower in CCA specimens compared with normal specimens in 16 out of the 20 pairs. B: miR-195 expression in normal biliary epithelial cell line H69 and 3 different human CCA cell lines. C: miR-195 expression in the more aggressive BDEneu cells is downregulated vs. related but less aggressive BDEsp cells. D, E: Co-culture of CCA cells with LX2 cells further downregulates miR-195 within cancer cells. F: Co-culture of CCA cells with LX2 cells reduces miR-195 levels within stellate cells (LX2).