FIG. 1.
Induction of RANTES expression in JEV-infected neuron/glia cultures. After 12 days in vitro, neuron/glia cultures were subjected to immunocytochemistry with antibodies against MAP-2, GFAP, and ED-1. Neuron/glia cultures before harvest were also photographed under phase contrast. Scale bar, 50 μm (A). Neuron/glia cultures were mock-infected or infected with JEV (NT113) over time. Total cellular RNAs were harvested and subjected to RT-PCR for the detection of RANTES and GAPDH (B). Culture supernatants were collected, and the concentration of RANTES was determined by enzyme-linked immunosorbent assay (n = 4) (C).