FIG. 7.
Target cell CypA regulates the cell-type-specific effects on HIV-1 replication. HIV-1-GFP transducing vectors, either wild type, CA mutant A92E, CA chimera NL4-3/CA9, or CA chimera NL4-3/MVP5180, were produced by transfection of 293T cells, normalized by RT activity, and used to infect Jurkat cells (A) or HeLa cells (B). The Jurkat cells were wild type (control) or homozygous for a deletion of the gene encoding CypA (Ppia−/−), as indicated (A). HeLa cells with wild-type levels of CypA (control) or with knocked-down CypA expression (CypA KD) were used, as indicated (B).