FIG. 1.
(A) Fluorescence microscopy analysis of Sf9 cells infected with astrovirus recombinant baculoviruses. Immunofluorescence of cells infected with AcNPV-ΔORF2 (panel 1), AcNPV-ORF2 (panel 2), and AcNPVRP8 (panel 4) with MAb 8E7 is shown. The GFP fluorescence of AcNPV-GFPΔORF2-infected cells is also shown (panel 3). (B) SDS-polyacrylamide gel (left) and Western blot (right) analysis of total cell extracts of cells infected with AcNPV-ΔORF2 (lanes 1), AcNPV-ORF2 (lanes 2), AcNPV-GFPΔORF2 (lanes 3), and AcNPVRP8 (lanes 4). Arrows indicate the molecular mass (in kilodaltons) of each of the recombinant products. The immunological detection was performed with a polyclonal anti-HAstV-1 antibody.