Fig. 1.
Ets-1 is required for efficient differentiation of αβ thymocytes. (A and B) Flow cytometric analysis of thymocytes from wild-type/RAG-2-/- (Wt → RAG) and Ets-1-/-/RAG-2-/- (Ets-1-/- → RAG) chimera mice. Numbers represent the percentage of cells found in the indicated quadrant or region. Histograms show the level of CD5 expression on CD4+CD8+ DP-gated thymocytes. Wild-type thymocytes are indicated by fine lines, and Ets-1-/- thymocytes are indicated by thick lines. (C) Analysis of thymus cell numbers. Thymus cell suspensions were counted, stained with anti-CD3-APC, anti-CD8-FITC, anti-CD4-bi, and anti-γδ-PE, and the percentages of triple-negative (CD4-CD8-CD3-), DP (CD4+CD8+), SP4 (CD4+), SP8 (CD8+), and γδ+ cells determined by FACS. Cell numbers were calculated by multiplying the total number of thymocytes by the percentage of each subset. Wild-type and Ets-1-/- subsets are indicated by open and shaded bars, respectively. These results are representative of six independent chimeras generated from different fetal liver cell injections. For each staining, potential RAG2-/--derived cells were gated out by using the Ly5.2 cell-surface marker. Heterozygous chimeras were identical to wild-type chimeras and are not shown.