FIG 3.
Repression of GADD45B results in lower levels of apoptosis markers in response to Nutlin-3 treatment. (A) Primary endothelial cells were transfected with miRNAs (24 h) and then treated with Nutlin-3 for 24 h. The protein expression of GADD45B and apoptosis markers was analyzed by Western blotting (n = 3). (B) Assays similar to those performed for panel A, but cells were transfected with siRNAs targeting GADD45B (n = 5). Graphs from biological replicates are shown on the right. *, P value of <0.05 compared to miNeg/siNeg without Nutlin-3; ‡, P value of <0.05 compared to miNeg/siNeg with Nutlin.